Genome-wide mining of gpx gene family provides new insights into cadmium stress responses in common carp (Cyprinus carpio)
Yaguo Xue 1, Lin Chen 2, Chuanju Dong 1, Xuejun Li 1 *, Peng Xu 2 *
1. College of Fishery, Henan Normal University, Xinxiang, Henan 453007, China
2. Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China
Glutathione peroxidase (Gpx) is one of the important members of antioxidant enzymes and plays an important role in regulating cell reactive oxygen species (ROS) homeostasis. Previous studies have revealed that the gpx gene family of common carp, Cyprinus carpio exhibited significant expansion as compared with the diploid related species Poropuntius huangchuchieni. Phylogenetic analysis showed that the gpx genes of common carp were clustered into five different subclades. In this study, we explored the effect of the supplement of Bacillus coagulans on the expression of gpx genes in the intestinal tract of allotetraploid carp under the stress of heavy metal cd2+, and separately added different concentrations of B. coagulans into the feed for feeding. The results showed that B. coagulans could activate the expression of most genes in gpx gene family at the concentration of 1.0×107 cfu/g and 1.0×108 cfu/g, and the gene expression was higher at the concentration of 1.0× 107 cfu/g. It was speculated that B. coagulans played a role in enhancing the antioxidant capacity of the organism, thus that effectively resisting the oxidative stress and diseases induced by reactive oxygen species.
Key words: Glutathione peroxidase(Gpx), phylogenetic analysis, cd2+, common carp, Bacillus coagulans
